LOCUS       sequence-129753-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control cobalamin riboswitch reporter vector for use in E. 
            coli; deletion of regulatory kissing loop interaction by conversion 
            of L5 to GAAA tetra loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Johnson JE Jr, Reyes FE, Polaski JT, Batey RT
  TITLE     B12 cofactors directly stabilize an mRNA regulatory switch.
  JOURNAL   Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 
            2012 Oct 14.
  PUBMED    23064232
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11607"; journalName: "Nature"; date: "2012-12-6- 6"; 
            volume: "492"; issue: "7427"; pages: "133-7"
COMMENT     Sequence Label: pENV8(GAAA)-GFPuv
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     575..603
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      complement(927..954)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggatctttc gaaagggcag attgtgtcga caggtaatgg ttgtctggta aaaggacagg
       61 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgg atccatcttc
      121 aatgttgtgg cgaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat
      181 gtatacattg tgtgagttat agttgtactc gagtttgtgt ccgagaatgt ttccatcttc
      241 tttaaaatca atacctttta actcgatacg attaacaagg gtatcacctt caaacttgac
      301 ttcagcacgc gtcttgtagt tcccgtcatc tttgaaagat atagtgcgtt cctgtacata
      361 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatccg gataacggga
      421 aaagcattga acaccataag agaaagtagt gacaagtgtt ggccatggaa caggtagttt
      481 tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc
      541 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      601 aactccagtg aaaagttctt ctcctttact cattttttct accggtaccc ggggatcctc
      661 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatc agcatgttga gtctccttgc
      721 tcgttgtatg gcctaggtgg cattcggagt ataaccgtat ctttcaatct ggacgaattt
      781 cacgtcactt tcccactacg cttttaggcc attattatat gaatatatta tacgagcctt
      841 atgcatgccc gtaaagttat ccagcaacca ctcatagacc tagggcagca gatagggacg
      901 acgtggtgtt agctgtggct agcgcaaaaa ggccatccgt caggatggcc ttctgcttaa
      961 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg
     1021 caacgttcaa atccgctccc ggcggatt
//
