LOCUS       sequence-129755-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP35
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             45..67
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             87..104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..140
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(144..152,153..167)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    883..903
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
       61 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      121 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      181 aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg gaactcgacg
      241 gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac gcgacgtatg
      301 ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg tggccgacgc
      361 tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac cacatgaaac
      421 agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg acgatctttt
      481 tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg gacacgctcg
      541 tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata ctcggccata
      601 agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag caaaagaacg
      661 ggatcaaagc caatttcaaa atccggcata acatagagga tgggggggtc caactggcgg
      721 atcactatca gcaaaacacg ccgatagggg atgggccggt cctcctcccg gataaccatt
      781 acctctcgta ccaaagcgcg ctctcgaagg acccgaatga gaaacgggac cacatggttc
      841 tcctggagtt cgtcacggcg gcgggcatag gtaccgatat cacaagtttg tacaaaaaag
      901 ctgaa
//
