LOCUS       sequence-129756-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses miR-146a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Chang TC, Yu D, Lee YS, Wentzel EA, Arking DE, West KM, Dang CV, 
            Thomas-Tikhonenko A, Mendell JT
  TITLE     Widespread microRNA repression by Myc contributes to tumorigenesis.
  JOURNAL   Nat Genet. 2008 Jan;40(1):43-50. Epub 2007 Dec 9.
  PUBMED    18066065
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2008-01"; volume: "40(1)"; pages: "43-50. Epub 2007 
            Dec 9"
COMMENT     Sequence Label: pMSCV-PIG-miR-146a
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        460..959
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(495..518)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     892..911
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(980..999)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cctttatcca gccctcactc cttctctagg cgccggaatt agatctctcg agagagatcc
       61 acccacatca gccttccaga ctgctggcct ggtctcctcc agatgtttat aactcatgag
      121 tgccaggact agacctggta ctaggaagca gctgcattgg atttaccagg cttttcactc
      181 ttgtatttta cagggctggg acaggcctgg actgcaagga ggggtctttg caccatctct
      241 gaaaagccga tgtgtatcct cagctttgag aactgaattc catgggttgt gtcagtgtca
      301 gacctgtgaa attcagttct tcagctggga tatctctgtc atcgtgggct tgaggacctg
      361 gagagagtag atcctgaaga actttttcag tctgctgaag agcttggaag actggagaca
      421 gaaggcagag tctcaggctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc
      481 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
      541 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
      601 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
      661 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
      721 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
      781 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
      841 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
      901 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
      961 cctgcagccc aagcttacca tgaccgagta caagcccacg gt
//
