LOCUS       sequence-129760-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses miR-26a-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Chang TC, Yu D, Lee YS, Wentzel EA, Arking DE, West KM, Dang CV, 
            Thomas-Tikhonenko A, Mendell JT
  TITLE     Widespread microRNA repression by Myc contributes to tumorigenesis.
  JOURNAL   Nat Genet. 2008 Jan;40(1):43-50. Epub 2007 Dec 9.
  PUBMED    18066065
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2008-01"; volume: "40(1)"; pages: "43-50. Epub 2007 
            Dec 9"
COMMENT     Sequence Label: pMSCV-PIG-miR-26a-2
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(149..151,152..154,155..868)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(194..215)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(455..474)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     802..823
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
       61 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      121 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg
      181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      241 tggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
      361 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      601 ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
      661 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      721 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      781 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      841 tctcggcatg gacgagctgt acaagtaatg aattaattaa gaattatcaa gcttatcgat
      901 accgtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag tctccagaaa
      961 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctt
//
