LOCUS       sequence-129769-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High-efficiency blue-shifted cation channelrhodopsin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL
  TITLE     Characterization of a highly efficient blue-shifted channelrhodopsin
            from the marine alga Platymonas subcordiformis.
  JOURNAL   J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 
            10.1074/jbc.M113.505495. Epub 2013 Aug 30.
  PUBMED    23995841
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.505495"; journalName: "J Biol Chem"; date: 
            "2013-10-11- 11"; volume: "288"; issue: "41"; pages: "29911-22"
COMMENT     Sequence Label: pcDNA3.1_PsChR2_EYFP
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..83)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(join(113..826,827..829,830..832,833..835))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(158..179)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     507..526
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     766..787
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggtttaaac tcaatggtga atggtgatga tgaccggtac gcgtagaatc gagaccgagg
       61 agagggttag ggataggctt accttcgaac cgcgggccct ctagagcata tgttacttgt
      121 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      181 gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt
      241 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      301 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
      361 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
      421 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      481 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      541 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      601 ggtcggggta gcgggcgaag cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc
      661 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
      721 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      781 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcact agcatttcca
      841 aagacttgcg cgtctggatg ccgcgctgct ccatgtcgtt cctcatcttc ataaagctcg
      901 cgcggttggc gtaagtctta gtcgagatgc gttcatcctc atcctcctcc tccttgtcca
      961 caaagcacga caccgtcacc tcacgcccga gggtgttcac cgtgatcggg cgtcgcacgt
     1021 ctccgtggat gataatatgt ttgtggatct cgagacgaag gaagtgtccg atcatcccc
//
