LOCUS       sequence-129771-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knock down expression of spliced Copia in Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     Nilson lab plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCoHygro L16_shRNA of spliced Copia
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(201..305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     373..391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(429..451)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     551..570
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     764..786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     778..795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     779..795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
       61 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
      181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
      361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
      421 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
      481 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      541 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      601 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
      661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      781 aaaacgacgg ccagtgccaa gcttaaaaag aaattcagag aatcaactgg tatttatccc
      841 aattaattct ctaaatttcg aagtattgag gaaaacatac ctatataaat gatcaacatc
      901 aggaaagagc agttgagaat tataagaatt ggcaaatggt cttaagaacc ctctgcttaa
      961 gattttcaaa atttccttta gaatcaaagt gtcctattgt tcgtttgttg aaa
//
