LOCUS       sequence-129774-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector can be used for homologous recombination and allows to 
            obtain recombinant neomycin resistant fluorescent cells that can 
            loose fluorescence after Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Gian Luca Forni
  TITLE     The plasmid is a modification of the p076 pPNT6 with the green 
            fluorescent protein gene cloned within the LoxP sites in xbaI site 
            next to Neomycin cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPNT6-eGFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..44)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        118..617
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(153..176)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     550..569
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     5'UTR           659..698
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 gtagaggcct ataacttcgt atagcataca ttatacgaag ttatgcgcag gatccccggt
       61 taacgcctta attaactcct cgagcatgct agcgtcgatt taaatgcgaa ttctaccggg
      121 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      181 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      241 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      301 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      361 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      421 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      481 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
      541 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
      601 catctccggg cctttcgacc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
      661 tcttggtggc gtgaaactcc cgcacctctt cggcaagcgc cttgtagaag cgcgtatggc
      721 ttcgtacccc tgccatcaac acgcgtctgc gttcgaccag gctgcgcgtt ctcgcggcca
      781 tagcaaccga cgtacggcgt tgcgccctcg ccggcagcaa gaagccacgg aagtccgcct
      841 ggagcagaaa atgcccacgc tactgcgggt ttatatagac ggtcctcacg ggatgggg
//
