LOCUS       sequence-129778-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 55
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(461..541)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      complement(636..722)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggcgataa gcttgtcgac
       61 ggatccgagc tcggtaccat ggatatctgc agttcttgcc atatgtatat ctccttctta
      121 aagttaaaca aaattatttc ccgggtgaac cgaattggga attcaggcat tgacgaaggc
      181 attcagctca ccatctttgt tgcgtgtgat gaatatcggc tgccggccca tctgcgtggt
      241 gtaatagtcg ttcttctcgg gaatctggct ctcgtgggcg agataaatcc agttgccctc
      301 aaagatgtgt ttcatctcta aatcgaacag ccgagggtcg gtgaacatct cgcgcttgca
      361 gcggtagatg ccctcgttct catcttcttc aacgagacta tctatatagt cgagccccag
      421 gtgcattgtc atggtcatga ctccattatt attgtttctg ttgcataaag cctaaggggt
      481 aggcctttct agagatagcc attttttgca ctcctgtatc cgcttcttgc aaggctggac
      541 ttatctctat caaattccct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
      601 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
      661 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      721 tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
      781 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
      841 caaattcgcg cggccgccac cgcggtggtc gtcgccgcac ttatgactgt cttctttatc
      901 atgcaactcg taggacaggg tgccggcagc gctctggg
//
