LOCUS       sequence-129779-        5634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 55
FEATURES             Location/Qualifiers
     source          1..5634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             999..1021
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1095..1114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1096..1114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1124..1141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1124..1140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1282..1737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1369..1388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1579..1600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1763..1867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1868..1936,1937..2728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2086..2105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3751..4701)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4749..4971
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcccaattcg gttcacccgg gaaataattt tgtttaactt taagaaggag
     1021 atatacatat ggcaagaact gcagatatcc atggtaccga gctcggatcc gtcgacaagc
     1081 ttatcgccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1141 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1201 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1261 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     1741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2341 tcgccttgat cgctgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag atctctgttc
     2881 gggggttcac tgacgctgcg tgactacgat ggtcaggttg cggaggctat ggccctggta
     2941 cgagcgctga acaaaatgac gaaagcaggt atgcctgaaa gcgtgcgtat tgcctgaaaa
     3001 cacaacccgc tacgggggag acttacccga aatctgattt attcaacaaa gccactttca
     3061 gtatggattt gggtgttacg ggggggggtt ggtgcttaaa atcgcgctga ggatttctga
     3121 tggtgttaag cgggcggttt tgagatgtaa actcgcccgt ttaacataat ggatcttgcg
     3181 cgcaccgccc gaacaccact cgccacaaaa aaccgccgga acgtccaaaa gtacgggttt
     3241 tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc gcagatccgg
     3301 cttcagccgg tttgccggct gaaagcgcta tttcttccag aattgccatg attttttccc
     3361 cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa gcagcatcgc
     3421 ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca ggtgttcaat
     3481 ttcatgttct agttgctttg ttttactggt ttcacctgtt ctattaggtg ttacatgctg
     3541 ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac caaaaactcg
     3601 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tatggacagt
     3661 tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     3721 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     3781 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
     3841 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     3901 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
     3961 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     4021 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     4081 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     4141 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     4201 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     4261 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     4321 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     4381 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     4441 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     4501 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     4561 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     4621 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     4681 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     4741 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
     4801 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     4861 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     4921 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     4981 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     5041 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     5101 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     5161 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     5221 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     5281 ttatggcggg tctgcggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg
     5341 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg
     5401 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg
     5461 tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa
     5521 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
     5581 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcg
//
