LOCUS       sequence-129780-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and crRNA 589 target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP568-3
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..345)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(360..386)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(387..407)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatgtataa taaagttgtg agactttttt cttggcggca caataaagtt tcctcttttt
       61 gttgggtcac tcaacgaaca caatacaaat atttgagcgc tcgcattttt ctcaaccttt
      121 cgggtttaaa gcacttcaaa atggaattga agagagattg tgaaagtaca tcagacagaa
      181 tcacattttg atagcattca cttcactcag atgcaaactt ttcagtgact ataaaaagtc
      241 ataaaataat aacattaaaa aagcaaataa attagcgaga gaattttttg acaaaaagaa
      301 agaagagtga tagagaagaa gggaatgctt gaaaggattt tgcatttatc actagtttag
      361 gcgtagtctg ggacgtcgta tgggtagacc ttacgcttct tctttggtgg tccggatcct
      421 ccgtctcctc cgagttggga gaggtcgata cgggtctcgt agagtccggt gatggattgg
      481 tggatgaggg tggcgtcgag gacctccttg gtggaggtgt aacgcttacg gtcgatggtg
      541 gtgtcgaagt acttgaaggc ggctggggct ccgaggttgg tgagggtgaa gaggtggatg
      601 atgttctcgg cttgctcacg gattggcttg tcacggtgct tgttgtaggc ggagaggacc
      661 ttgtcgaggt tggcgtcggc gaggatgaca cgcttggaga actcggagat ttgctcgatg
      721 atctcgtcga ggtagtgctt gtgttgctcg acgaagagtt gcttttgctc gttgtcctct
      781 ggggatccct tgagcttctc gtagtgggag gcgaggtaga ggaagttgac gtacttggat
      841 gggagggcga gctcgtttcc cttttggagc tctccggcgg aggcgagcat acgcttacgt
      901 ccgttctcga gctcgaagag ggagtacttt gggagcttga tgatgaggtc cttcttgacc
      961 tccttgtatc ccttggcctc gaggaagtcg attgggttct tctcgaagga ggaacgctcc
     1021 atgatggtga ttccgaggag ctccttgacg gactt
//
