LOCUS       sequence-129781-         435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 
            over-expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC147
FEATURES             Location/Qualifiers
     source          1..435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..35
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19..35
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    44..100
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(104..121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(104..120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattcg agctcggtac
       61 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
      121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      241 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
      301 ttcagtttag cttcatggag tcaaagattc aaatagagga cctaacagaa ctcgccgtaa
      361 agactggcga acagttcata cagagtctct tacgactcaa tgacaagaag aaaatcttcg
      421 tcaacatggt ggagc
//
