LOCUS       sequence-129783-       10605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 
            over-expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC147
FEATURES             Location/Qualifiers
     source          1..10605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..253
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    275..299
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1599..2228
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2662..3729
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3795..3989
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4225..4247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4333..4473
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4388..4407
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4488..4505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4659..5247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4739..4758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5334..6128)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6553..6577
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6655..6829
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6886..7683)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6997..7016)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7607..7626
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7746..8423)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8614..8635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8650..8655,8656..8673,8674..8680)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8688..8704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8693..8715
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8712..8728
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8712..8728
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8729..8956
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    8737..8793
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8797..8814)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8797..8813)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8806..8828)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        9190..9535
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
       61 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
      121 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
      181 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
      241 atgttactag atcgggaatt aaactatcag tgtttgacag gatatattgg cgggtaaacc
      301 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
      361 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
      421 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
      481 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
      541 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
      601 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
      661 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
      721 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
      781 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
      841 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
      901 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
      961 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     1021 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     1081 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     1141 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     1201 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     1261 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     1321 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     1381 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     1441 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     1501 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     1561 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     1621 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     1681 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     1741 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     1801 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     1861 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     1921 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     1981 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     2041 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     2101 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     2161 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     2221 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     2281 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     2341 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     2401 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     2461 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     2521 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     2581 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     2641 agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     2701 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     2761 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     2821 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     2881 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     2941 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     3001 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     3061 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     3121 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     3181 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     3241 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     3301 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     3361 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     3421 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     3481 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     3541 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     3601 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     3661 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     3721 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     3781 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     3841 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     3901 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     3961 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     4021 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     4081 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     4141 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     4201 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4261 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4321 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     4381 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     4441 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     4501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     5101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
     5341 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     5401 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     5461 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     5521 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     5581 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     5641 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     5701 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     5761 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
     5821 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
     5881 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
     5941 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
     6001 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
     6061 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
     6121 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     6181 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     6241 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     6301 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     6361 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     6421 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     6481 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     6541 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
     6601 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg
     6661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     6721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     6781 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     6841 cgatcgactc tagctagagg atcgatccga accccagagt cccgctcaga agaactcgtc
     6901 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     6961 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     7021 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     7081 attttccacc atgatattcg gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc
     7141 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     7201 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     7261 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     7321 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     7381 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     7441 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     7501 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     7561 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     7621 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatccc
     7681 catggtcgat cgacagatct gcgaaagctc gagagagata gatttgtaga gagagactgg
     7741 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg
     7801 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     7861 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     7921 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     7981 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     8041 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     8101 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     8161 ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     8221 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     8281 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     8341 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     8401 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     8461 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
     8521 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8581 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8641 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8701 acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga
     8761 tcctctagag tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg
     8821 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     8881 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     8941 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
     9001 tagcttcatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     9061 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     9121 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     9181 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     9241 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     9301 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     9361 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     9421 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     9481 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     9541 gactcttgac agtagtataa tatattatca aattattatg aacagtacat ctatgtcttc
     9601 attgggagtg agaaaaggtt catggactga tgaagaagat tttcttctaa gaaaatgtat
     9661 tgataagtat ggtgaaggaa aatggcatct tgttcccata agagctggta actattaaat
     9721 taactatcac gttattttta tttgtctttc tgtctcattt tatttgacgt tattacgaat
     9781 atcatctgaa aatgtacgtg caggtctgaa tagatgtcgg aaaagttgta gattgaggtg
     9841 gctgaattat ctaaggccac atatcaagag aggtgacttt gaacaagatg aagtggatct
     9901 cattttgagg cttcataagc tcttaggcaa caggcatgca agtttatgtt ttgacaaaat
     9961 ttgattagta tatattatat atacgtgtga ctatttcatc taaatgttac gttattttac
    10021 gtagatggtc acttattgct ggtagacttc ccggaaggac agctaacgat gtgaaaaact
    10081 attggaacac taatcttcta aggaagttaa atactactaa aattgttcct cgcgaaaaga
    10141 ttaacaataa gtgtggagaa attagtacta agattgaaat tataaaacct caacgacgca
    10201 agtatttctc aagcacaatg aagaatgtta caaacaataa tgtaattttg gacgaggagg
    10261 aacattgcaa ggaaataata agtgagaaac aaactccaga tgcatcgatg gacaacgtag
    10321 atccatggtg gataaattta ctggaaaatt gcaatgacga tattgaagaa gatgaagagg
    10381 ttgtaattaa ttatgaaaaa acactaacaa gtttgttaca tgaagaaata tcaccaccat
    10441 taaatattgg tgaaggtaac tccatgcaac aaggacaaat aagtcatgaa aattggggtg
    10501 aattttctct taacttacca cccatgcaac aaggagtaca aaatgatgat ttttctgctg
    10561 aaattgactt atggaatcta cttgattaac agctcgaatt tcccc
//
