LOCUS       sequence-129790-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon 
            based on Bean Yellow Dwarf Virus; sgRNA= 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC223
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             301..321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atctctgagt tctctaaaag ggtgatcctc gctgatgcaa acctcgataa ggtgttgtct
       61 gcttacaaca agcacagaga taagcctatc agggaacagg cagagaacat catccatctc
      121 ttcaccctta ccaacctcgg tgctcctgct gctttcaagt acttcgatac aaccatcgat
      181 aggaagagat acacctctac caaagaagtg ctcgatgcta ccctcatcca tcagtctatc
      241 actggactct acgagactag gatcgatctc tcacagctcg gtggtgattc aagggctgat
      301 cctaagaaga agaggaaggt ttgactcgag atatgaagat gaagatgaaa tatttggtgt
      361 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
      421 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
      481 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
      541 tgtgctatgg natggactat ggaatatgat taaagataag gagctccggt gacggaccca
      601 tggcttcgtt gaacaac
//
