LOCUS       sequence-129791-         345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology 
            arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon 
            based on Bean Yellow Dwarf Virus; sgRNA= 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC223
FEATURES             Location/Qualifiers
     source          1..345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttttttgtt tatcagctta cattttcttg aaccgtagct ttcgttttct tctttttaac
       61 tttccattcg gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg
      121 catcgaacct tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa
      181 tcttcaaaag gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga
      241 acaatagtat ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat
      301 cgcttagata agaaaacgaa gctgagttta tatacagcta gagtc
//
