LOCUS       sequence-129800-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing the GUS enzyme. Can be fused with linker to 
            the C-terminal end of protein of interest. For use in plants and 
            compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-GUS-OcsT
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     67..84
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     68..84
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(155..278)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctatagggcg aattgggccc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat
       61 tcgatttgta aaacgacggc cagtcttaag ctcgggccct gcagctctag agctcgaatt
      121 ctacaggtca ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt
      181 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      241 attttacgtt tctcgttcaa ctttcttgta caaagtgggg atccaagctt cgggaggtat
      301 gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt
      361 cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga
      421 aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg
      481 taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg
      541 ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa
      601 tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta
      661 tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca
      721 gactatcccg ccgggaatgg tgattaccga cgaa
//
