LOCUS       sequence-129815-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB216
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..231
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    308..432
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(575..594)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
       61 gaagcacatc aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt
      121 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      181 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      241 caccccggtg aacagctcct cgcccttgct caccatgctc ccaccggagc ctccagcgct
      301 gttatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa
      361 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      421 ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc
      481 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
      541 cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc
      601 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
      661 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaa
//
