LOCUS       sequence-12982-e        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Jnk3a1
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             35..57
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 anggagtgnc ggatacattc ccctctgaaa taattttgtt taactttaag aaggagatat
       61 acatatgcac catcatcatc atcattcttc tggtgtagat ctgggtaccg agaacctgta
      121 cttccaatcc aatggcagca aaagcaaagt tgacaaccag ttctacagtg tggaagtggg
      181 agactcaacc ttcacagttc tcaagcgcta ccagaatcta aagcctattg gctctggggc
      241 tcagggcata gtttgtgccg cgtatgatgc tgtccttgac agaaatgtgg ccattaagaa
      301 gctcagcaga ccctttcaga accaaacaca tgccaagaga gcgtaccggg agctggtcct
      361 catgaagtgt gtgaaccata aaaacattat tagtttatta aatgtcttca caccccagaa
      421 aacgctggag gagttccaag atgtttactt agtaatggaa ctgatggatg ccaacttatg
      481 tcaagtgatt cagatggaat tagaccatga gcgaatgtct tacctgctgt accaaatgtt
      541 gtgtggcatt aagcacctcc attctgctgg aattattcac agggatttaa aaccaagtaa
      601 cattgtagtc aagtctgatt gcacattgaa aatcctggac tttggactgg ccaggacagc
      661 aggcacaagc ttcatgatga ctccatatgt ggtgacacgt tattacagag cccctgaggt
      721 catcctgggg atgggctaca aggagaacgt ggatatatgg tctgtgggat gcattatggg
      781 agaaatggtt cgccacaaaa tcctctttcc aggaagggac tatattgacc agtggaataa
      841 ggtaattgaa caactaggaa caccatgtcc agaattcatg aagaaattgc aacccacagt
      901 aagaaactat gtggagaatc ggcccaagta tgcgggactc accttcccca aactcttccc
      961 agattccctc ttcccagcgg actccgagca caataaactc aaagccagcc aagccaggga
     1021 cttgttgtca aagatgctag tgattgaccc agcaaaaaga atatcagtgg acgacgcctt
     1081 acagcatccc tacatcaacg tctggtatga cccagccgaa gtggaggcgc ctccacctca
     1141 gatatatgac aagcagttgg atgaaagaga acacacaatt gaagaatgga aagaacttat
     1201 ctacaaggaa gtaatgaatt cagaatgaca ttggaagtgg ataacggatc cgaattcgag
     1261 ctccgtcgac agctgcggcg cctcggc
//
