LOCUS       sequence-129820-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing nosT. Necessary to complete the 
            transcriptional reporters by providing the poly-A tail. For use in 
            plants and compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-nosT
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     62..79
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     63..79
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(150..273)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      280..532
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(562..657)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(694..713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(695..713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(718..734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(718..734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(815..833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(816..833)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 ttgtaaaacg acggccagtc ttaagctcgg gccctgcagc tctagagctc gaattctaca
      121 ggtcactaat accatctaag tagttggttc atagtgactg catatgttgt gttttacagt
      181 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      241 acgtttctcg ttcaactttc ttgtacaaag tggggtagcg atcgttcaaa catttggcaa
      301 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
      361 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
      421 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
      481 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
      541 atcccccctc gacagaagct tcaactttat tatacaaagt tggcattata aaaaagcatt
      601 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga
      661 gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta ttacatggtc
      721 atagctgttt cctgaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag
      781 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttg
//
