LOCUS       sequence-129821-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing nosT. Necessary to complete the 
            transcriptional reporters by providing the poly-A tail. For use in 
            plants and compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-nosT
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..59
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    120..215
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(245..497)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    504..627
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(698..714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      731..740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      735..744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(775..793)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(800..816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catatggtcg acctgcaggc ggccgcgaat tcactagtga ttcaggaaac agctatgacc
       61 atgtaatacg actcactata ggggatatcg cggccgcgtt aacgctacca tggagctcca
      121 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
      181 cttttttata atgccaactt tgtataataa agttgaagct tctgtcgagg ggggatcaat
      241 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
      301 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
      361 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
      421 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
      481 caaatgtttg aacgatcgct accccacttt gtacaagaaa gttgaacgag aaacgtaaaa
      541 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      601 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta
      661 gaattcgagc tctagagctg cagggcccga gcttaagact ggccgtcgtt ttacaaatcg
      721 aattcccgcg gccgccatgg cggccgggag catgcgacgt cgggcccaat tcgccctata
      781 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
//
