LOCUS       sequence-129828-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB402
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..440)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(237..256)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
       61 aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata
      121 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
      181 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
      241 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
      301 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
      361 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
      421 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
      481 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
      541 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
      601 atatatgcct tgagcaa
//
