LOCUS       sequence-12983-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: MKK4
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..33
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             138..161
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatg
       61 attacccaga tctgggtacc gagaacctgt acttccaatc caatgcggct ccgagcccgg
      121 atccggcagc caacatggac tataaggacg atgacgacaa acagggtaaa cgcaaagcac
      181 tgaagttgaa ttttgcaaat ccacctttca aatctacagc aaggtttact ctgaatccca
      241 atcctacagg agttcaaaac ccacacatag agagactgag aacacacagc attgagtcat
      301 caggaaaact gaagatctcc cctgaacaac actgggattt cactgcagag gacttgaaag
      361 accttggaga aattggacga ggagcttatg gttctgtcaa caaaatggtc cacaaaccaa
      421 gtgggcaaat aatggcagtt aaaagaattc ggtcaacagt ggatgaaaaa gaacaaaaac
      481 aacttcttat ggatttggat gtagtaatgc ggagtagtga ttgcccatac attgttcagt
      541 tttatggtgc actcttcaga gagggtgact gttggatctg tatggaactc atgtctacct
      601 cgtttgataa gttttacaaa tatgtatata gtgtattaga tgatgttatt ccagaagaaa
      661 ttttaggcaa aatcacttta gcaactgtga aagcactaaa ccacttaaaa gaaaacttga
      721 aaattattca cagagatatc aaaccttcca atattcttct ggacagaagt ggaaatatta
      781 agctctgtga cttcggcatc agtggacagc ttgtggactc tattgccaag acaagagatg
      841 ctggctgtan gccatacatg gcacctgaaa gaatagaccc aagcgcatca cgacaaggat
      901 atgatgtccg ctctgatgtc tggagtttgg ggatcacatt gtatgagttg gccacaggcc
      961 gatttcctta tccaaagtgg aatagtgtat ttgatcaact aacacaagtc gtgaaaggag
     1021 at
//
