LOCUS       sequence-129837-       13913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB331
FEATURES             Location/Qualifiers
     source          1..13913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             3522..3530
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3676..3795
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     terminator      3820..4072
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4106..4122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4106..4122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4130..4146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4154..4160,4161..4178,4179..4184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4199..4220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4305..4480
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6564..7240)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6607..6627)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7435..7459
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7980..8771
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9017..9605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9506..9525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9759..9776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9791..9931
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9857..9876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10017..10039
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10275..10469
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10535..11608)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12037..12666)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggagagaga gaagaacgtg gtgtacggcc ccgagcccaa gcaccctctg
     2101 ggcaacttca ccgccggcga gatgctgtac aacgctctgc acaagcactc ccacatcccc
     2161 caggccatcc tggacgtgat gggcaacgag tccctttcct accaggagtt cttcgacact
     2221 actgtgaagc tgggccagag cctccagaac tgtggctaca agatgaacga tgtcgtgtcg
     2281 atctgtgcag agaacaacaa gagattcttc atccccatca tctccgcctg gtacatcggc
     2341 atggtggtgg cccctgtgaa cgaggactat atcccagacg agctgtgtaa agtgaccggc
     2401 atctccaagc cgatcctggt cttcaccact aggaagatcc tgcctaaggt tttggaggtt
     2461 aaagacagaa ccaactacat aaagaggatc atcatactgg actctgaaga gaacctgctg
     2521 ggctgcgaga gcctgcacaa cttcatgtcc aggtactccg acaacaacct ccaaacattc
     2581 aagcctctgc actacgaccc tgtggaccag gtagccgcca tcctgtgctc ctccggcaca
     2641 accggcctgc ctaaaggcgt gatgcagacc cacaggaaca tctgtgtgag actcacacac
     2701 gcatctgacc ccagagtggg tacacaactc atccccggcg tatccgtgct ggcctacctg
     2761 ccattcttcc acgccttcgg cttcagtatc aacctgggct atttcatggt gggcctgaga
     2821 gtggtgatgc tccgaaggtt taaccaggag gtgttcctga aggccatcca ggactacgag
     2881 gtgaggagcg tgatcaacgt tccctccaca atcctgttcc tgtccaagag ccctctggtg
     2941 gacaagtacg acctatccac cctggcggag ctgtgctgtg gagccgctcc tctggcgaag
     3001 gaggtggccg agatcgccgt gaagaggctg aacctgccag ggatacggtg tggctacggt
     3061 ctaacagagt ctacctccgc caacatccat actctgcaca acgagttcaa gtccggctcc
     3121 ctgggcaagg tgacacctta catggccgcc aagatcatcg acaggaacac cggcgaggcc
     3181 ctgggtccaa accaggtggg cgagctgtgc atctggggac ctatggtaac aaaaggctat
     3241 gtgaacaacc cacaggctac taaggaggcc atcgacgacg acggctggct gcactctggc
     3301 gacttcggct actacgacga ggacgagtat ttctacatcg tggaccggta caaggagctg
     3361 atcaaataca agggctatca ggtcgcccct gtggagctgg aggagatcct ccttcagcac
     3421 ccaggcatca gggacgtggc cgtcgtgggt atccctgaca tcgaggccgg cgagctgcca
     3481 gccggcttcg tggtgaagca gcccggcgcc caactcaccg ctaaggaggt gtacgacttc
     3541 ctggcccaga gggtgtctca ctccaagtac ctgaggggcg gcgtaaggtt cgtggactct
     3601 atccccagga acgtgacagg caagattagt cgaaaagagc tgagggaggc cctgatggag
     3661 aaggcttcta agcttagcca tggcttcccg ccggaggtgg aggagcaggc tgctggcacg
     3721 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
     3781 gctaggatca atgtgtaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     3841 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3901 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3961 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4021 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4081 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4201 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     4261 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     4321 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     4381 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaaat
     4441 tcccttatct gggaactact cacacattat tatggagaaa ctcgaagtta gtatatagtc
     4501 cttccatcac cctctgtgat aatctcctta aaacttgctc ccccaggaat cataggcaga
     4561 acgtgctcct ggtggggaac tatcacatcc agtaaataag gaccgggaga atctagcata
     4621 gtttgaattg ctgctcttag ttcatccttc ttcaccacac gcgcatttgg caccttgaaa
     4681 ccgtctgcaa ctgtacagaa gtcggggtag atctgctcct cgtgatctgg atcacctaag
     4741 taagtatgtg ctctgttggc cttgtagaat ctatcctcca attgtacgac cattcctaga
     4801 tgttggttgt ttaagatcat gatcttcacg ggtaacttct ccacaaaaat tgtagccaac
     4861 tcctgtacgt tcatcacaaa gctaccatcg ccatcgatgt cgatcacagg aatatctggt
     4921 tgagttgcgg cagcccccaa agcagaagga agtccgaatc ccatggctcc aagacctcca
     4981 gatgtcaacc aacgccgcgg ttcgttgtac ttgaaccact gggctgccca catttgatgt
     5041 tggccaacac cagtagatat gatggcattt ccaccagtga gttcgtacag cgtctgtatg
     5101 gcagattgag gagcaatggc atcctcgaat acgggaaact tcattggcca tttctgcttt
     5161 tgttcttcta gttctgccac ccagttggag aagtcgaaag tcctcttttg actcttcaag
     5221 agggcattga tttcacttag cgcaggtttc acatcagcac acatggacac atggggctgt
     5281 ttgttctttc caatttcagc agggtcaata tcgatatgaa cgatgctgga cctgctagca
     5341 aaagtctcca gtttaccagt cactcgatcg tcgaaacgca ctccaaatgc caacaacaga
     5401 tctgcttggt ccacggcgta gttggcgtaa acagttccat gcattccaag cattttcaaa
     5461 cttaatggat cagactcggg atacgctccc aaacccatta aagtcgatgt gataggaatt
     5521 ccggtaagct cgacaaattc cctcaactct tcgctggcgt gattgcaacc tcctccagaa
     5581 tacactacag gcttcttaga actagaaatg agtcttataa tctgctcgat taacgttggt
     5641 tccggtggcc gaggcagtcg ttgtaagtag cccttcagtt gcattggctg agaccaatct
     5701 ggaatagcca tttgctgttg cacgtccttg ggaatatcta ccaagactgg gcccggccta
     5761 cctgaagctg ccaaaaagaa agcctccctg attacgcgag ggatgtcatc aatgttcata
     5821 acaaggtaat tgtgcttggt gatcgatctc gtaacctcca caattggagt ttcttgaaaa
     5881 gcatcggtac caatcatcct actggatact tgacctgtaa tggccactag tggcacactg
     5941 tccaagaggg catcagcgag cccggtaaca agattggtcg ctccaggacc ggatgtggct
     6001 atacaaaccc ctactcgtcc agacgattta gcataacctt cagcagcgaa aatctccccc
     6061 tgctcgtggc gacagagaat gttgcggatg ctgctggaac gtgtaagagc ctggtgaatc
     6121 tccatagacg ctccgccggg ataagcgaac accgttctaa tcccttctcg ctcaagagcc
     6181 tccaccaaaa tgtcggctcc tttgcggggc tcgtcatcct tgtagcgaga ctgaaagtcg
     6241 gacggggttg gtgcaacgag tgacttcccg ctgctaagcc gtggtccact cacatcgact
     6301 gcagactgga tcttctcgct aacagcttgg gctttcactt ccagtgaacg ctcagtcttc
     6361 cgagaggtgt tgtatgcatg tcccctcaga gcggctcccg agaagaaacc actcctcacc
     6421 tttagctctt tagctccctt gcaacaagac cttgccgatg gctgcacctt ctccaggctc
     6481 gctatgctca aaggcacagc tgcgcatcca ctaattgacg ccatgctcga gagagataga
     6541 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
     6601 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     6661 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     6721 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     6781 gcctttcctt tatcgcaatt atggcatttg taggtgccac cttccttttc tactgtcctt
     6841 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     6901 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     6961 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
     7021 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     7081 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     7141 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     7201 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     7261 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag
     7321 ctgctctagc caatacgcaa accgaattca gaaataaatt cagcctaatt cggcgttaat
     7381 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     7441 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     7501 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     7561 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     7621 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     7681 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     7741 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     7801 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     7861 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     7921 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     7981 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     8041 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     8101 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     8161 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     8221 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     8281 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     8341 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     8401 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     8461 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     8521 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     8581 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     8641 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     8701 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     8761 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     8821 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     8881 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     8941 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     9181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9781 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     9841 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     9901 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     9961 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
    10021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
    10081 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
    10141 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
    10201 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
    10261 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
    10321 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
    10381 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
    10441 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
    10501 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
    10561 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
    10621 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
    10681 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
    10741 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
    10801 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
    10861 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
    10921 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
    10981 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    11041 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    11101 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    11161 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    11221 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    11281 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    11341 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    11401 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    11461 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    11521 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    11581 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    11641 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    11701 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    11761 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    11821 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    11881 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    11941 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    12001 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    12061 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    12121 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    12181 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    12241 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    12301 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    12361 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    12421 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    12481 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    12541 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    12601 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    12661 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    12721 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    12781 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    12841 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    12901 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    12961 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    13021 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    13081 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    13141 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    13201 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    13261 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    13321 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    13381 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    13441 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    13501 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    13561 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    13621 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    13681 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    13741 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    13801 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    13861 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
