LOCUS       sequence-129842-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB329
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..126
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(163..168,169..186,187..193)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(313..332)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
       61 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      121 actatggcgg cccctcgagg ggccgcatta ggcaccccag gctttacact ttatgcttcc
      181 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      361 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      421 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      481 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      541 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      601 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      661 attgagaata tgtttttcgt ctcagccaat ccctgg
//
