LOCUS       sequence-129847-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of murine Tfap2c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-Tfap2c
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4444..5032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4497..4517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4915..4926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5035..5051)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5036..5052)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5557..5737
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5763..5780)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5769..5993
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6039..6467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6126..6145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6336..6357
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6476..6496)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6481..6810
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6661..6796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6723..6742
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6858..6905
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6924..7298
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7428..7549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7465..7484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7519..7538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7598..7614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7598..7614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7611..7633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7622..7638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7646..7652,7653..7670,7671..7676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7691..7712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7829..7846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8000..8588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8080..8099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8759..9550,9551..9619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9382..9401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9620..9724)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg gcttggacgc
     3061 catgttgtgg aaaataacag ataatgtcaa gtatgaagag gattgcgagg atcgccacga
     3121 ctcgagcagt aatggcaacc ctcgcatccc tcacctctcc tctccgggac aacatctcta
     3181 cagtcccgcg ccgcctctct cgcacaccgg ggttgcagag taccagccgc ctccttactt
     3241 cccgccgcct taccagcagc tggcatactc gcagtccgcc gaccattact cgcatctggg
     3301 agaggcttac gctgccgcca tgaaccccct gcaccagcct gcggccaccg gcagccagca
     3361 acaggcctgg ccgggtcgac agagtcagga gggctctagc ctggcctcgc accacagccg
     3421 ctctgcaagt ctaatacccc atatttcagg gctggagggg ggctcggtga gcgcccggcg
     3481 ggaagtctac cgccggtccg acctgctgct gcctcacgcg cacgccctgg aagccggcct
     3541 ggctgagaac ctggggctgc acgagatggc tcaccccata gaggaggtgc agaatgtgga
     3601 cgacgcgcac ttgctcctac acgatcagac tgtcattcgc aaaggaccca tttcgatgac
     3661 caagaaccct ttggggctcc cttgccagaa ggacctggtg ggagtggtca tgaaccccag
     3721 tgaggtcttc tgctcggtcc ctggaagact gtccctgctc agctccacgt cgaagtacaa
     3781 agtaactgtg gctgaggtac agaggcgact gtcaccaccg gaatgcctaa acgcctcgct
     3841 cctgggaggt gtgctcagaa gagcaaagtc caaaaatgga ggccggtcct tgagggagaa
     3901 gttggacaaa attggattga accttccggc cgggagacgg aaagctgccc acgtcactct
     3961 cctcacgtct ctcgtggaag gtgaagccgt ccacctagca cgggacttcg cctatgtctg
     4021 cgaagctgag ttccctagta aagcggtggc tgactattta acgagaccac atcttggggg
     4081 acggaatgag atggccacgc ggaagagtat gttgttggct gcacagcagg tgtgcaagga
     4141 gttcactgac cttctccatc aagatcggac acccaacggg aacaacaggc ccgcccaggt
     4201 cttggagccg aacatacaaa actgtttgtc tcatttcagc ctgataactc atggctttgg
     4261 cagccaggcc atctgtgcgg cggtctccgc agtgcagaat tatatcaagg aggctctaat
     4321 cgccatcgat aagtcctaca tgaacccggg ggaccagagt ccggctgatt ccagcaagac
     4381 gatggagaaa atggaaaagc acaggaagta ataaaagccg aattcgatat caagcttatc
     4441 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4501 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4561 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4621 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4681 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4741 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4801 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4861 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4921 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4981 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     5041 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     5101 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     5161 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5221 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5281 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5341 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5401 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5461 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     5521 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     5581 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5641 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5701 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     5761 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5821 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5881 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5941 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6001 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6181 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6241 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6301 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6361 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6421 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6481 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6541 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6601 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6661 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6721 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6781 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6841 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     6901 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     6961 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     7021 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     7081 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     7141 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     7201 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7261 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7321 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7381 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7501 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7561 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9721 cgcgcacatt tccccgaaaa gtgccacctg ac
//
