LOCUS       sequence-129848-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB333
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..222
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    278..402
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(545..564)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcacat
       61 caggccgtag ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      121 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      181 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      241 gaacagctcc tcgcccttgc tcaccatgct gttatcaacc actttgtaca agaaagctga
      301 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      361 gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc
      421 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      481 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      541 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      601 gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      661 gcccgggcgt gtcaataata tcactctgta catccaca
//
