LOCUS       sequence-129853-        8940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Feb 15. 26(7):2000-9."
COMMENT     Sequence Label: pCI-SEP-NRI
FEATURES             Location/Qualifiers
     source          1..8940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1414..2124
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1441..1469)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2064..2084
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    6054..6175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6065..6084)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6119..6138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6356..6811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6443..6462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6653..6674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6878..6897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6997..7019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7057..7075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7143..7247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7248..7316,7317..8108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7466..7485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8279..8867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8768..8787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattccg ttgctgtcga
     1081 atattggttt gttaaggcag tttctgtaga ggtttctaag agaccagtcg cgcagtccgc
     1141 gctgctgtcc tttccgcctt ttccgcgcgg gtgttcgagc agcgccaaac acgcttcagc
     1201 acctcggaca gcatccgccg cgctcgcccg gggctcctag agaacccggg ggcgcttgac
     1261 cgcgcgcggg cggcccgcgg gtcgtacatc gcgaggtcgt cgcactcgcg caacccagag
     1321 ccaggcccgc tgtgcccgga gctcatgagc accatgcacc tgctgacatt cgccctgctt
     1381 ttttcctgct ccttcgcccg cgccgccact aggagtaaag gagaagaact tttcactgga
     1441 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     1501 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     1561 ggaaaactac ctgttccttg gccaacactt gtcactactt taacttatgg tgttcaatgc
     1621 ttttcaagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
     1681 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1741 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1801 aaagaagatg gaaacattct tggacacaaa ttggaataca actataacga tcaccaggtg
     1861 tacatcatgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tagacacaac
     1921 attgaagatg gaggcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1981 gggcccgtcc ttttaccaga caaccattac ctgtttacaa cttctactct ttcgaaagat
     2041 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2101 catggcatgg atgaactata caaagctagt tgcgacccca agatcgtcaa catcggcgcg
     2161 gtgctgagca cgcgcaagca tgaacagatg ttccgcgagg cagtaaacca ggccaataag
     2221 cgacacggct cttggaagat acagctcaac gccacttctg tcacccacaa gcccaacgcc
     2281 atacagatgg ccctgtcagt gtgtgaggac ctcatctcta gccaggtcta cgctatccta
     2341 gttagccacc cgcctactcc caacgaccac ttcactccca cccctgtctc ctacacagct
     2401 ggcttctaca gaatccctgt cctgggactg actacccgaa tgtccatcta ctctgacaag
     2461 agtatccacc tgagtttcct tcgcacggtg ccgccctact cccaccagtc cagcgtctgg
     2521 tttgagatga tgcgagtcta caactggaac cacatcatcc tgctggtcag cgacgaccac
     2581 gagggacggg cagcgcagaa gcgcttggag acgttgctgg aggaacggga gtccaaggca
     2641 gagaaggtgc tgcagtttga cccaggaacc aagaatgtga cggctctgct gatggaggcc
     2701 cgggaactgg aggcccgggt catcatcctt tctgcaagcg aggacgacgc tgccacagtg
     2761 taccgcgcag ccgcaatgct gaacatgacg ggctctgggt acgtgtggct ggtcggggaa
     2821 cgcgagatct ctgggaacgc cctgcgctac gctcctgatg gcatcatcgg acttcagctc
     2881 atcaatggca agaatgagtc agcccacatc agtgacgccg tgggcgtggt ggcacaggca
     2941 gttcacgaac tcctagagaa ggagaatatc actgacccac cgcggggttg cgtgggcaac
     3001 accaacatct ggaagacagg accattgttc aagagggtgc tgatgtcttc taagtatgcg
     3061 gacggagtga ctggccgtgt ggaattcaat gaggatgggg accggaagtt tgccaactat
     3121 agtatcatga acctgcagaa ccgcaagctg gtgcaagtgg gcatctacaa tggtacccat
     3181 gtcatcccaa atgacaggaa gatcatctgg ccaggaggag agacagagaa acctcgagga
     3241 taccagatgt ccaccagact aaagatagtg acaatccacc aagagccctt cgtgtacgtc
     3301 aagcccacaa tgagtgatgg gacatgcaaa gaggagttca cagtcaatgg tgacccagtg
     3361 aagaaggtga tctgtacggg gcctaatgac acgtccccag gcagcccacg ccacacagtg
     3421 ccccagtgct gctatggctt ctgcatagac ctgctcatca agctggcgcg gaccatgaat
     3481 tttacctatg aggtgcacct ggtggcagat ggcaagtttg gcacacagga gcgggtaaac
     3541 aacagcaaca aaaaggagtg gaacggaatg atgggcgagc tactcagtgg ccaagcggac
     3601 atgattgtgg caccactgac catcaacaat gagcgtgcgc agtacataga gttctccaag
     3661 cccttcaagt accagggcct gaccattttg gtcaagaagg agattcccag gagcacactg
     3721 gactcattta tgcagccttt tcagagcaca ctgtggttgc tagtaggact gtcagttcat
     3781 gtggtggctg tgatgctgta cctgctggac cgcttcagtc cctttggccg attcaaggtg
     3841 aacagtgagg aggaggagga agatgcactg accctgtcct ctgccatgtg gttttcctgg
     3901 ggcgtcctgc tcaactccgg cattggggaa ggtgcccccc ggagtttctc tgcacgtatc
     3961 ctaggcatgg tgtgggctgg tttcgccatg atcatagtgg cttcctacac tgccaacttg
     4021 gcagctttcc tggtgctgga tcggcctgag gagcgcatca cgggcatcaa tgaccccagg
     4081 ctcagaaacc cctcagacaa gttcatctac gcaactgtaa agcagagctc cgtggacatc
     4141 tacttccgga ggcaggtgga gttgagtacc atgtaccggc acatggaaaa acacaattac
     4201 gagagcgcag ctgaggccat ccaggctgtg cgggacaaca agctgcacgc ctttatctgg
     4261 gactcggccg tgctggagtt tgaggcttca cagaagtgcg atctggtgac cacgggtgag
     4321 ctgttcttcc gctcaggctt tggcatcggc atgcgcaagg acagcccctg gaagcagaac
     4381 gtttccctgt ccatactcaa gtcccatgag aatggcttca tggaagatct ggataagaca
     4441 tgggttcggt atcaggaatg cgactcccgc agcaatgctc ctgcaaccct cacttttgag
     4501 aacatggcag gggtcttcat gctggtggct ggaggcatcg tagctgggat tttcctcatt
     4561 ttcattgaga tcgcctacaa gcgacacaag gatgcccgta ggaagcagat gcagctggct
     4621 tttgcagccg tgaacgtgtg gaggaagaac ctgcaggata gaaagagtgg tagagcagag
     4681 cccgacccta aaaagaaagc cacatttagg gctatcacct ccaccctggc ctccagcttc
     4741 aagagacgta ggtcctccaa agacacgagc accgggggtg gacgcggcgc tttgcaaaac
     4801 caaaaagaca cagtgctgcc gcgacgcgct attgagaggg aggagggcca gctgcagctg
     4861 tgttcccgtc atagggagag ctgagacgcc ccgcccgccc tcctctgccc ctcccccgca
     4921 gacagacgca cgggacagcg gcctggccca cgcagagccc cggagcacga cggggtcggg
     4981 ggaggagcac tcccagcctc ccccaggccg tgcccgcctg cccaccggtc ggccggctgg
     5041 ccggtccacc ctgtcccggc cccgcgcgtg cccccgacgt cggagctaac gggccgcctt
     5101 gtctgtgtat ttctatttta cagcagtacc atcccactga tatcacgggc ccgctcaacc
     5161 tctcagatcc ctcggtcagc accgtggtgt gaggcccccc ggaggcgccc acctgcccag
     5221 ttagcccggc caaggacact gatgagtcct gctgctcggg aaggcctgag ggaagcccac
     5281 ccgccccaga gactgcccac cctgggcctc ccgtccgcct gctctgctgc ctggcgggca
     5341 gcccctgcag gaccaaggtg cggaccagag cggctgagga tgggccagag ctgagccggc
     5401 tgggcagggc cacagggcgc tccggcagag gcagggccct gaggtctctg agcagtgggg
     5461 tgaggggcct aagtggcccc ggtcggagga gtctggagca gaaatggcag ccccatcctt
     5521 cctccagcca ctaccccaag ctacagtggg ggcctatggc cccagcttgc taggtcaccc
     5581 ccgacccttc ctccagcgcc tgctctctgc aacttgattt ccacctctct cctgctgcac
     5641 caccctccca cgacatttcc ccaccccatt cactgggttg tctctgacct ttcccagggc
     5701 tagccttcac tgccctagtg gcagtgcttc aggggtgctt tctggctccc agacatctag
     5761 ggctccagac tccaagaggg ctgagccttc tcttctgtcc gcagccacaa taggcttcct
     5821 cagacgctgg ctcgtgatga gtcccgcacc ttgggcacca gggagcgcca tctgcctccc
     5881 agtccggtgt cactcacccc actaccttgt acatgaccag ctctcccagt gtcccagtgt
     5941 ctgccccagg gacaccgggc gcgcacagcc acccctaatc ccggtattca gtggtgatgc
     6001 ctaaaggaat gtcagaaaaa aaaaaaaaaa gcggccgctt cgagcagaca tgataagata
     6061 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6121 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     6181 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     6241 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgggctggc gtaatagcga
     6301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     6361 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6421 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6481 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6541 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6601 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6661 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6721 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6781 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     6841 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     6901 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7021 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7081 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7621 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
