LOCUS       sequence-129868-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB227
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..170
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(211..516)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(313..332)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atcagctcgc tcatgctccc accggagcct ccagcgctgt tatcaaccac tttgtacaag
       61 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      121 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
      181 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      241 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      301 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      361 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
      421 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
      481 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
      541 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
      601 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
      661 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
      721 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
      781 tatattctta taccgcaaaa
//
