LOCUS       sequence-129871-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-mCherry
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..761)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(329..348)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     598..616
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattcg gctttcctcc ttacttgtac
       61 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
      121 gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc
      181 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
      241 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
      301 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
      361 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
      421 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      481 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
      541 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg
      601 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
      661 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
      721 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tatatataag ccgaattcgg
      781 ggccgcgga
//
