LOCUS       sequence-129879-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene 
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Long S, Wang Q, Sibley LD
  TITLE     Analysis of non-canonical calcium dependent protein kinases in 
            Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9.
  JOURNAL   Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15
  PUBMED    26755159
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15"
COMMENT     Sequence Label: pLoxP-DHFR-mCherry
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(3..36)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccataacttc gtatagcata cattatacga agttataagc ttcgccaggc tgtaaatccc
       61 gtgagtcgtc ctcacaaatc atcaagcagg tgtcctcagg gagactgcct gactgagtta
      121 tgctaattcc tttctacttt ggcgtggtca cgggcgcgcc ggatccttaa ttaagtctag
      181 catgtcattc gattttcacc ccccgcgtag ttcctgtgtg tcattcgttg tcgagacaac
      241 tctgtcccgc cccggtgctg ttccatatgc gtgactttcc cgcaattttt tcagactttc
      301 aggaaagaca ggctccggaa cgatctcgtc catgactggt aaatccacga caccgcaatg
      361 gcccccagca cctctatctc tcgtgccagg ggactaacgt tgtatgcgtc tgcgtcttgt
      421 ctttttgcat tcgctttcca aaaaagagag ccatccgttc ccccgcacat tcaacgccgc
      481 gagtgcggtt tttgtctttt ttgagtggta ggacgctttt catgcgcgaa ctacgtggac
      541 attaagttcc attctctttt tcgacagcac gaaaccttgc attcaaaccc gcccgcggaa
      601 gatccgatct tgctgctgtt cgcagtccca gtagcgtcct gtcggccgcg ccgtctctgt
      661 tggtgggcag ccgctacacc tgttatctga ctgccgtgcg cgaaaatgac gccatttttg
      721 ggaaaatcgg ggaacttcat tctttaaaag tatgcggagg tttccttttt cttctgttcg
      781 tttctttttc tcgggtttga taaccgtgtt cgatgtaagc actttccgtc tctcctccgt
      841 gctttgttcg acatcgagac caggtgtgca gatccttcgc
//
