LOCUS       sequence-129883-       13638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB136
FEATURES             Location/Qualifiers
     source          1..13638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2045..2734
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    2750..2874
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2911..2916,2917..2934,2935..2941)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2995..3654
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3061..3080)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3996..4301
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4180..4199
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4342..4466)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4498..4750
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4784..4800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4784..4800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4797..4819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4808..4824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4832..4838,4839..4856,4857..4862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4877..4898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4983..5157
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5198..6223)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6290..6965)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7160..7184
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7705..8496
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8742..9330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9231..9250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9484..9501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9516..9656
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9582..9601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9742..9764
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10000..10194
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10260..11333)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11762..12391)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      361 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      421 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      481 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      541 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      601 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      661 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      721 ttcacttata ctaatatttg gtcaacttca agagagatta gaacttcgtt ctccaaagtg
      781 taaaggccga caagcagaaa aggaaacttc aatcagaagc cttctaggtt ttcccgcgtc
      841 ccaaaaagcg aagggaggac ggggaggacg gtgaggccag aagaagaaag caacagtaaa
      901 atctcatgtg attgcgtgca tgtcatggat ctatgggatg ctagtccacc gtttggaagt
      961 atgtgcagaa ggagaaaggc gcgggaggcc aaaagcgact tcggccacct ctctactttc
     1021 tagaaagttt gctcgcagct tgtaataagc gcggaaaagg tactcgaaaa accggagcaa
     1081 tcggtgtggt atcagcagtc gggttcgcta aacctacgtc ttcttatttt ggaatgttaa
     1141 attgaaaggg cgtgccggcc ctaagcacgg acgatgtgac agacggagga gtgtgagcaa
     1201 ttcggatgac gatgctggcc ggcagtcacg acaaagagtc cgaaggggca tggacagggc
     1261 aatatataaa tggaaaagag gctttgaata ttcctaaccc ttcgtcgtcg gcctcccaac
     1321 ttgccctcgc atgctgaatt ggccgcctat ctgcgaatgg ccggccgccg accctaagtc
     1381 gcaataactt gaacggagcc gatgcaaagt ccttatcgaa cgctcgttat tataaacggg
     1441 gcagacctag gtatttaatt atatcaaaac cctccgcaat ttcgctaatc attccctaat
     1501 ttcttctact ttggcgtcta gtgctgtggt cgcgcttgct ctcaaagctc gctgggaggc
     1561 agtggagcgt ctggcttaaa atcgatcgcc gcaaccctag agttcctttc acttctttct
     1621 ctcttgcgtg gagagcatcg acatcgaaga gctgcatctt gtcttctggt atgcgccttg
     1681 tgccctcgtc tctgtacgtc gttacgattg ctcgatattg tgattgtcta gacctgaaac
     1741 ccgactggag cttttacgtg gtgtcgattg gggcctcgag cgagtgattt tttaggttcg
     1801 ttttagccaa gtacatccca ctttggtcag tcctgttatc tcggtaaggt gttcagttcg
     1861 gtctctggtg cggaggtgcc tcgtaagttt cctgcatgat agatttgtct atccttgact
     1921 tgaagaggag tcgaaggtca gcgaggttgg ctgtgtgcta tggacgtcgg tgctccttgg
     1981 ttccgtgctt ctatgggtgt tgcctcaaca gtattgatgt gcctgcagaa aggttgagct
     2041 tacaatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     2101 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     2161 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     2221 taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt
     2281 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     2341 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     2401 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
     2461 ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga
     2521 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag
     2581 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact
     2641 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc
     2701 cagatactgc gacctcccta gcaaactggg gcacaagcta gagttatcaa caagtttgta
     2761 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     2821 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     2881 cctcgagggg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     2941 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     3001 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     3061 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     3121 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     3181 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     3241 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     3301 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     3361 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     3421 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     3481 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     3541 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     3601 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct
     3661 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     3721 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     3781 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     3841 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     3901 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     3961 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     4021 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     4081 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtcccccgtg
     4141 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     4201 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     4261 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     4321 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     4381 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     4441 cgttcagctt tcttgtacaa agtggttgat aacagcgctt agagctcgca tttccccgat
     4501 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     4561 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     4621 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     4681 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     4741 ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag ctgtttcctg
     4801 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4861 aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg cgcgcctatt
     4921 tctgaattca acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
     4981 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
     5041 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
     5101 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
     5161 gagcttgtcg atcgacagat cccggtcggc atctactcta tttctttgcc ctcggacgag
     5221 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     5281 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     5341 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     5401 agagttggtc aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct
     5461 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     5521 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     5581 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     5641 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     5701 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     5761 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     5821 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga
     5881 ccggttgtag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     5941 aacggcggga gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg
     6001 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     6061 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     6121 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     6181 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg
     6241 atctgcgaaa gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc
     6301 tctccaaatg aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg
     6361 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     6421 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     6481 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     6541 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     6601 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     6661 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     6721 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     6781 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     6841 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     6901 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     6961 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     7021 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccga attcagaaat
     7081 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     7141 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     7201 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     7261 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     7321 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     7381 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     7441 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     7501 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     7561 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     7621 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     7681 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     7741 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     7801 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     7861 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     7921 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     7981 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     8041 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     8101 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     8161 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     8221 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     8281 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     8341 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     8401 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     8461 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     8521 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     8581 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     8641 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc
     8701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8881 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9361 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9421 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9481 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     9541 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     9601 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     9661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     9721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     9781 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     9841 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     9901 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     9961 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
    10021 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
    10081 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
    10141 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
    10201 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
    10261 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
    10321 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
    10381 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
    10441 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
    10501 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
    10561 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
    10621 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
    10681 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
    10741 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
    10801 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
    10861 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
    10921 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
    10981 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
    11041 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
    11101 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
    11161 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
    11221 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
    11281 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
    11341 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
    11401 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    11461 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    11521 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    11581 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    11641 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    11701 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    11761 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    11821 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    11881 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    11941 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    12001 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    12061 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    12121 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    12181 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    12241 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    12301 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    12361 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    12421 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    12481 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    12541 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    12601 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    12661 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    12721 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    12781 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    12841 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    12901 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    12961 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    13021 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    13081 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    13141 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    13201 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    13261 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    13321 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    13381 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    13441 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    13501 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    13561 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    13621 ggacgaacgg ataaacct
//
