LOCUS       sequence-129887-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2189
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..49)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atctgatatc acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc
       61 tcgagagctc gctgtgaaga tcccagcaaa ggcttacaaa gtgttatctc ttttgagact
      121 tgttgagttg aacactggtg ttttcatcaa acttaccaag gacgtgtacc cattgttgaa
      181 acttgtatca ccatatattg ttatcggaca accttcactt gcatctatcc gttctttaat
      241 ccaaaagaga tctagaataa tgtggcaaag gccagaagat aaagaaccaa aagagataat
      301 cttgaatgac aacaatatcg ttgaagagaa attaggtgat gaaggtgtca tttgtatcga
      361 ggatatcatc catgagattt cgacgttggg cgaaaatttc tcgaaatgta ctttcttcct
      421 attaccattc aaattgaaca gagaagtcag tggattcggt gccatctccc gtttgaataa
      481 actgaaaatg cgcgaacaaa acaaggagac tcgtcaaatt tcaaacgctg ccacggctcc
      541 agttatccaa gtagatatcg actcaatgat ttccaagttg aattgattaa ctataaaagg
      601 aaaatatctg tacaatagac atcgggctcc cattggccct acccacatat gtagaaatac
      661 attactctat tcactactgc atttagttat gtttaacatt tgatatagca gactaccgcc
      721 aggcacaata tattcccctt ccctcttgcc attcgctgta cttgtggtgg attccaattc
      781 agcgcagtca cgtgctagta atcaccgcat ttttttcttt tcctttcagg ctaaaaccgg
      841 ttccgggcct gatccctgca ctcattttct aacggaaaac cttcagaagc ataactaccc
      901 attccagttt agagtcatga caggttcaac atcagatgct tcatatactt ttatatattg
      961 aattatataa atatatctat gtactctaag taagtacatc tgctttaacg cattcctaca
     1021 tttgcttcga tttattttta ttgttgatac ctatttgaag aagtaaaaag tatcc
//
