LOCUS       sequence-129888-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP11-clathrin light chain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pEGFP-GFP11-Clathrin light chain
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..142
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tgtcgggagg
       61 ttccggtgga agcggaggta gcggcggatc catgcgtgac cacatggtcc ttcatgagta
      121 tgtaaatgct gctgggatta caggcggatc cggactcaga tctcgagctc aagcttcgaa
      181 ttctgcagtc gacatggctg atgactttgg cttcttctcg tcgtcggaga gtggtgcccc
      241 ggaggcggcg gaggaggacc cggcggccgc cttcctggcc cagcaggaga gcgagattgc
      301 aggcatagag aacgacgagg gcttcggggc acctgccggc agccatgcgg cccccgcaca
      361 gccgggcccc acgagtgggg ctggttctga ggacatgggg accacagtca atggagatgt
      421 gtttcaggag gccaacggtc ctgctgatgg ctacgcagcc attgcccagg ctgacaggct
      481 gacccaggag cctgagagca tccgcaagtg gcgagaggag cagaggaaac ggctgcaaga
      541 gctggatgct gcatctaagg tcacggaaca ggaatggcgg gagaaggcca agaaggacct
      601 ggaggagtgg aaccagcgcc agagtgaaca agtagagaag aacaagatca acaaccgggc
      661 atccgaggag gctttcgtga aggaatccaa ggaggagacc ccaggcacag agtgggagaa
      721 ggtggcccag ctatgtgact tcaaccccaa gagcagcaag cagtgcaaag atgtgtcccg
      781 cctgcgctcg gtgctcatgt ccctgaagca gacgccactg tcccgctagg gatccaccgg
      841 atctagataa ctgatcataa tcagccatac c
//
