LOCUS       sequence-129890-        9964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP53
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1420..1799
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1800..2003
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1954..1974
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2005..2023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2026..2044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2054..2078
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2151..2160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2160..2183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2196..2222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2238..2262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5287..5311)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5330..5829
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5365..5388)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5762..5781
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5850..6449
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5850..5869)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6346..6366
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7035..7051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7059..7065,7066..7083,7084..7089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7104..7125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7242..7259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7413..8001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7493..7512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8172..8963,8964..9032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8795..8814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9033..9137)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9205..9223
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9261..9283)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9383..9402
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9596..9618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9746..9765
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt
     1441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1501 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1561 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1621 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1681 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1741 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1801 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1861 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1921 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1981 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
     2041 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     2101 gttttgacct ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg
     2161 actacaagga tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg
     2221 ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg catggtttgt cagggagatg
     2281 cctgtatatt ttgtgcattg aagacgatat ttgcacagtt ccaacacagt cgagaaaaag
     2341 cacttccctc agataacata aggcatgctc ttgcagaaag tttcaaagat gagcagcgat
     2401 ttcaacttgg ccttatggat gatgctgcgg agtgctttga aaatatgttg gagaggattc
     2461 attttcacat agtgccaagc agagatgcag acatgtgtac ctctaaatct tgtatcactc
     2521 accagaagtt tgctatgact ctgtatgaac agtgtgtgtg tcgtagctgt ggagcatcgt
     2581 cagatcctct accttttaca gaatttgtgc ggtacatttc tacaacagcc ttatgcaatg
     2641 aggttgaaag aatgttggaa aggcatgaac gctttaaacc tgaaatgttt gcagaattgc
     2701 tacaagcagc aaatacaaca gatgactata ggaaatgtcc tagtaactgt ggccaaaaaa
     2761 taaaaattcg ccgtgtttta atgaattgcc cagagattgt tacaattggt ttagtctggg
     2821 actccgagca ttctgacttg accgaagctg ttgttcggaa tctagcaaca catctttatc
     2881 ttcctgggct tttttataga gttactgatg aaaatgccaa aaatagtgaa cttaaccttg
     2941 ttggtatgat ctgctacacc agccaacatt attgtgcctt tgcatttcac accaaaagtt
     3001 ccaaatgggt attttttgat gatgcaaatg tgaaagagat tggaactaga tggaaagatg
     3061 ttgtctccaa atgcattcga tgccactttc agccactact tttgttttat gcaaacccag
     3121 atggcacagc agtttctact gaggatgcac tcaggcaggt catcagctgg tcacattaca
     3181 aatctgttgc agaaaatatg ggatgtgaaa agcctgtaat tcataagtca gataatttaa
     3241 aagaaaatgg atttggtgat caggcaaagc agagagaaaa tcagaaattt ccaactgata
     3301 atatttcatc atctaatcgg agccacagtc acacaggtgt agggaaagga ccagctaagt
     3361 taagtcacat tgatcaaagg gaaaagataa aagacatttc cagagaatgt gctctgaaag
     3421 ctattgaaca gaaaaactta ctttcttcac aaaggaaaga tttagagaag ggacaaagaa
     3481 aagatttagg acgacataga gatttggttg atgaagacct ttcacatttc caatctggat
     3541 cacctcctgc cccaaatggt tttaaacaac atgggaatcc acatctatat catagtcaag
     3601 gaaaaggatc atataaacat gaccgagttg tacctcagag tcgagcttct gcacaaataa
     3661 taagttcaag taaatcccag attcttgctc caggagagaa aataactggc aaagttaaga
     3721 gtgacaatgg cactggatat gacacagaca gcagccaaga ttctagggat agaggaaaca
     3781 gctgtgatag cagcagtaaa agccggaacc gaggttggaa acctatgaga gaaacattaa
     3841 atgttgatag tatttttagt gaaagtgaaa aaagacagca tagtccaaga cataaaccaa
     3901 atatcagtaa taagcctaaa tctagcaagg atccgagttt tagtaattgg ccaaaagaga
     3961 atccaaagca aaaaggttta atgaccatat atgaagatga aatgaagcag gaaataggaa
     4021 gcagaagttc ccttgaatct aatggaaaag gagcagagaa aaataaaggc cttgtagagg
     4081 gtaaagtgca tggtgataat tggcagatgc aaaggactga gtctggatat gaaagcagtg
     4141 atcacatcag taatggttct actaatttgg actcacctgt tatcgatgga aatggtacag
     4201 taatggatat cagtggtgtt aaagaaacag tatgcttcag tgaccagatt acgacaagca
     4261 acctaaataa agaacgtggg gactgtacct cccttcagag ccaacatcac ttagaaggct
     4321 ttagaaaaga actcaggaat ttggaagcag gctataaatc tcatgaattc cacccagaat
     4381 cacatttaca aataaaaaat catttgataa aaagatcaca tgtacatgaa gacaatggaa
     4441 agttatttcc ttcatccagt ctacaaatac ccaaggacca taatgcaaga gaacatatcc
     4501 accagtcaga tgaacagaaa cttgaaaaac cgaatgaatg caaattttct gagtggctta
     4561 atatagaaaa ttctgagaga acaggtttgc cttttcacgt tgataactct gcttctggga
     4621 agagagtgaa cagtaatgaa ccatcttcat tatggtcttc acacctaaga actgttgggt
     4681 taaagccaga aactgctcct ctcatccagc aacaaaatat catggatcaa tgttactttg
     4741 agaactctct atccacagaa tgtataattc ggtcagccag cagatctgat gggtgtcaga
     4801 tgccaaaact tttttgccag aatctaccac cccctttgcc accaaagaaa tatgctataa
     4861 ccagtgtgcc acagtcagag aaaagcgaat ctacacctga tgtcaaactt acagaggtgt
     4921 ttaaagctac ctctcatctt ccgaagcaca gtttaagtac agcttcagaa ccaagtttag
     4981 aagtgagtac acatatgaat gatgaaagac ataaagaaac atttcaagtg agagaatgtt
     5041 ttggcaacac accaaactgt ccatccagct cctcaactaa cgattttcag gcaaactcag
     5101 gtgccattga tgcattttgc caaccagaac tagactctat ttctacctgt ccaaatgaga
     5161 cagtttcatt aactacctat ttttcagttg atagctgcat gacggataca tatagattga
     5221 aataccatca gaggcccaag ctctcttttc cagagagcag tggcttttgt aataattcac
     5281 tatcttagcc aactttcttg tacaaagtgg ttcgataacg aattctaccg ggtaggggag
     5341 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     5401 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     5461 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     5521 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     5581 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     5641 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     5701 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     5761 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     5821 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg
     5881 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5941 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6001 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6061 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6121 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6181 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6241 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6301 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6361 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6421 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     6481 aaaggagcgc acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa
     6541 aggggggaat gaaagacccc acctgtaggt ttggcaagct agagaaccat cagatgtttc
     6601 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     6661 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     6721 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
     6781 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     6841 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
     6901 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
     6961 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
     7021 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8341 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8401 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8461 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8521 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8581 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8641 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8701 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8761 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8821 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8881 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8941 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9001 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9061 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9121 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9181 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     9241 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9301 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9361 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9421 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     9481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9601 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
     9661 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     9721 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     9781 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
     9841 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     9901 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     9961 gggg
//
