LOCUS       sequence-129894-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP11x7-mCherry-alpha-tubulin in insect cells. 5 a. a. 
            linker lengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pACUH-GFP11x7-mCherry-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..453
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     primer_bind     complement(611..629)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctgaatag ggaattggga attcatgcgt gaccacatgg tccttcatga gtatgtaaat
       61 gctgctggga ttacaggtgg ctctggaggt agagatcata tggttctcca cgaatacgtt
      121 aacgccgcag gcatcactgg cggtagtgga ggacgcgacc atatggtact acatgaatat
      181 gtcaatgcag ccggaataac cggagggtcc ggaggccggg atcacatggt gctgcatgag
      241 tatgtgaacg cggcgggtat aactggtggg tcgggcggac gagaccatat ggtgcttcac
      301 gaatacgtaa acgcagctgg cattactggc ggatcaggtg gcagggatca catggtactc
      361 catgagtacg tgaacgctgc tggaatcaca ggcggtagcg gcggtcggga ccatatggtc
      421 ctgcacgaat atgtcaatgc tgccggtatc accggcggca aattcatggt gagcaagggc
      481 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      541 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      601 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      661 ctgtcccctc agttcatgta cggctccaag gcctacgtga a
//
