LOCUS       sequence-129895-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP11x7-mCherry-alpha-tubulin in insect cells. 5 a. a. 
            linker lengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pACUH-GFP11x7-mCherry-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..295)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          425..490
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gcgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg
      121 cactatacat caaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
      181 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
      241 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
      301 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
      361 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
      421 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
      481 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
      541 agaagtaagg ttccttcaca aagatcctct agattagtac tcctcagcgc cctcaccctc
      601 gccgtcaccg gagtccatgc cgacctcctc gtagtccttc tcgagggcag ccaaatcctc
      661 acgggcctcg gagaactctc cctcctccat accctcacca acgtaccagt ggacgaaggc
      721 acgcttggcg tacatcagat cgaa
//
