LOCUS       sequence-129896-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pcDNA3.1-GFP(1-10)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             142..783
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     primer_bind     complement(839..856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg
      121 gtggaattcg ttaacagatc tatgtccaaa ggagaagaac tgtttaccgg tgttgtgcca
      181 attttggttg aactcgatgg tgatgtcaac ggacataagt tctcagtgag aggcgaagga
      241 gaaggtgacg ccaccattgg aaaattgact cttaaattca tctgtactac tggtaaactt
      301 cctgtaccat ggccgactct cgtaacaacg cttacgtacg gagttcagtg cttttcgaga
      361 tacccagacc atatgaaaag acatgacttt tttaagtcgg ctatgcctga aggttacgtg
      421 caagaaagaa caatttcgtt caaagatgat ggaaaatata aaactagagc agttgttaaa
      481 tttgaaggag atactttggt taaccgcatt gaactgaaag gaacagattt taaagaagat
      541 ggtaatattc ttggacacaa actcgaatac aattttaata gtcataacgt atacatcact
      601 gctgataagc aaaagaacgg aattaaagcg aatttcacag tacgccataa tgtagaagat
      661 ggcagtgttc aacttgccga ccattaccaa caaaacaccc ctattggaga cggtccggta
      721 cttcttcctg ataatcacta cctctcaaca caaacagtcc tgagcaaaga tccaaatgaa
      781 aaaggaacag gtggcggcgg aagttagtct agagggcccg tttaaacccg ctgatcagcc
      841 tcgactgtgc cttctagttg ccagccatct gttgttt
//
