LOCUS       sequence-129897-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pcDNA3.1-GFP(1-10)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..668)
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     primer_bind     complement(756..775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(757..775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(820..864)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 gactaacttc cgccgccacc tgttcctttt tcatttggat ctttgctcag gactgtttgt
       61 gttgagaggt agtgattatc aggaagaagt accggaccgt ctccaatagg ggtgttttgt
      121 tggtaatggt cggcaagttg aacactgcca tcttctacat tatggcgtac tgtgaaattc
      181 gctttaattc cgttcttttg cttatcagca gtgatgtata cgttatgact attaaaattg
      241 tattcgagtt tgtgtccaag aatattacca tcttctttaa aatctgttcc tttcagttca
      301 atgcggttaa ccaaagtatc tccttcaaat ttaacaactg ctctagtttt atattttcca
      361 tcatctttga acgaaattgt tctttcttgc acgtaacctt caggcatagc cgacttaaaa
      421 aagtcatgtc ttttcatatg gtctgggtat ctcgaaaagc actgaactcc gtacgtaagc
      481 gttgttacga gagtcggcca tggtacagga agtttaccag tagtacagat gaatttaaga
      541 gtcaattttc caatggtggc gtcaccttct ccttcgcctc tcactgagaa cttatgtccg
      601 ttgacatcac catcgagttc aaccaaaatt ggcacaacac cggtaaacag ttcttctcct
      661 ttggacatag atctgttaac gaattccacc acactggact agtggatccg agctcggtac
      721 caagcttaag tttaaacgct agccagcttg ggtctcccta tagtgagtcg tattaatttc
      781 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
      841 ccaccgtaca cgcctaccgc ccattt
//
