LOCUS       sequence-129920-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of murine Id2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-Id2
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(612..636)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(633..671)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    704..722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttgattatcg ataagcttga tatcgaatta tctgcagaat tcggctttgc catttattta
       61 gccacagagt actttgctat cattcgacat aagctcagaa gggaattcag atgcctgcaa
      121 ggacaggatg ctgatgtccg tgttcagggt ggtcagcggc gtcctggacg cctggttctg
      181 tccaggtctc tggtgatgca ggctgacgat agtgggatgc gagtccaggg cgatctgcag
      241 gtccaagatg taatcgatga cgtgctgcag gatttccatc ttggtcacct tcttgttctg
      301 ggggatgctg ggcaccagtt ccttgagctt ggagtagcag tcgttcatgt tgtagagcag
      361 actcatcggg tcgtccaccg gggttttgct ccgggagatg cccaagctgt ggtccgacag
      421 gctgtttttc ctaacggacc tcaccggact gaaggctttc atgctgctcg taggaaagcc
      481 gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca agctaattcg
      541 gggccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg
      601 cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt
      661 acacgcctac ctcgacccgg gtaccgagct cgactttcac ttttctctat cactgatagg
      721 gagtggtaaa ctcgactttc acttttctct atcactgata gg
//
