LOCUS       sequence-129926-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tetracycline/doxycycline dependent expression
            of murine Elf5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Kubaczka C, Senner CE, Cierlitza M, Arauzo-Bravo MJ, Kuckenberg P, 
            Peitz M, Hemberger M, Schorle H
  TITLE     Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts.
  JOURNAL   Cell Stem Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005.
  PUBMED    26412560
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 22. pii: S1934-5909(15)00360-4. doi: 
            10.1016/j.stem.2015.08.005."
COMMENT     Sequence Label: pLV-tetO-Elf5
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2663..2681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2705..2723
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2747..2765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2789..2807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2831..2849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2873..2891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2924..2961
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2958..2982
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3921..4509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3974..3994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4392..4403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4512..4528)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4513..4529)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5034..5214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5240..5257)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5246..5470
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5516..5944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5603..5622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5813..5834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5953..5973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5958..6287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6138..6273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6200..6219
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6335..6382
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6401..6775
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6905..7026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6942..6961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6996..7015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7075..7091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7075..7091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7088..7110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7099..7115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7123..7129,7130..7147,7148..7153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7168..7189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7306..7323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7477..8065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7557..7576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8236..9027,9028..9096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8859..8878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9097..9201)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccggaggtta gtgctatcag tgatagagaa
     2641 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2701 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2761 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2821 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2881 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgagtaggcg tgtacggtgg
     2941 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     3001 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaatta
     3061 tctgcagaat tcggcttctt cacggtgatg ttggactccg taacccatag caccttcctg
     3121 cccaacgcat ccttctgtga ccccctgatg ccttggaccg atctgttcag caatgaagac
     3181 tactaccccg cctttgagca tcagacagcc tgtgattcct actggacatc agtgcaccct
     3241 gaatactgga ccaagcgcca tgtctgggaa tggctccaat tctgctgtga ccagtacaag
     3301 cttgatgcca actgcatctc cttctgtcac ttcaacatca gcggcctgca gctctgcagc
     3361 atgacgcagg aggagttcat tgaggcagcc ggcatctgtg gggagtacct gtacttcatt
     3421 ctccagaaca ttcgctcgca aggttactcc tttttcaatg atgctgaaga aaccaagact
     3481 ggcatcaaag actatgctga ttccagttgc ttgaaaacaa gtggcatcaa gagtcaagac
     3541 tgtcacagcc gaacaagcct ccaaagttct cacctgtggg aatttgtcag agacttgctg
     3601 ctgtcccctg aagagaactg tggcatcctg gaatgggaag acagggagca gggcattttc
     3661 cgagtggtta agtcagaagc cctggcaaag atgtggggac aaaggaagaa gaatgacagg
     3721 atgacgtacg agaagctgag ccgagccctg agatactact ataaaacggg aattctggag
     3781 cgggttgacc ggaggttagt gtacaaattt ggaaagaacg cgcacgggtg gcaggaagag
     3841 aaactctgat ggacacaagc cgaattccag cacactggcg gccgttacta gtggatcaat
     3901 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3961 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4021 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4081 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4141 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4201 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4261 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4321 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4381 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4441 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4501 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     4561 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     4621 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     4681 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4741 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     4801 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     4861 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     4921 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     4981 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5041 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5101 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5161 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     5221 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5281 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5341 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5401 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5461 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5521 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5581 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5641 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5701 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5761 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5821 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5881 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5941 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6001 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6121 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6181 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6241 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6301 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     6361 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     6421 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     6481 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     6541 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     6601 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     6661 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     6721 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     6781 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6841 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6901 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6961 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7021 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7081 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7141 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7201 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7261 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7321 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7381 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7441 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7501 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7921 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7981 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8041 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
