LOCUS       sequence-129932-         316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For making transgenic fish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D
  TITLE     Haematopoietic stem cells derive directly from aortic endothelium 
            during development.
  JOURNAL   Nature. 2010 Mar 4;464(7285):108-11. doi: 10.1038/nature08738. Epub 
            2010 Feb 14.
  PUBMED    20154733
REFERENCE   2  (bases 1 to 316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08738"; journalName: "Nature"; date: "2010-03-4- 4"; 
            volume: "464"; issue: "7285"; pages: "108-11"
COMMENT     Sequence Label: bactin2:loxP-STOP-loxP-DsRed-express
FEATURES             Location/Qualifiers
     source          1..316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    198..231
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      262..271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc
       61 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgcgc accttgaagc
      121 gcatgaactc cttgatgacg tcctcggagg aggccatggt ggcgaccggt ggatcccggg
      181 cccgcggtac cgtcgacata acttcgtata atgtatgcta tacgaagtta ttaggtccct
      241 cgacctgcag acttactcaa gccatggtgg ctagcttgcg gaattccaga catgataaga
      301 tacattgatg agtttg
//
