LOCUS       sequence-129933-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For making transgenic fish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D
  TITLE     Haematopoietic stem cells derive directly from aortic endothelium 
            during development.
  JOURNAL   Nature. 2010 Mar 4;464(7285):108-11. doi: 10.1038/nature08738. Epub 
            2010 Feb 14.
  PUBMED    20154733
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08738"; journalName: "Nature"; date: "2010-03-4- 4"; 
            volume: "464"; issue: "7285"; pages: "108-11"
COMMENT     Sequence Label: bactin2:loxP-STOP-loxP-DsRed-express
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(380..385,386..403,404..410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     423..445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     442..458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     442..458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     500..520
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        502..520
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(530..541)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    join(553..564,565..727)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgtag
      481 ggataacagg gtaatgcgcg caattaaccc tcactaaagg gaacaaaagc tggagctccc
      541 aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag
      601 tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag
      661 taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa
      721 gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa
      781 atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga
      841 cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag
      901 tagaagctga ttctcaaatt ggttagtgtc aagcctagct cttttggggc tgaaaagcaa
      961 tcctgc
//
