LOCUS       sequence-129952-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pcDNA3.1-sfCherry(1-10)
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     540..558
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(796..840)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 gactcgagcg gccgccactg tgctggatat ctgcagaatt ccaccacact ggactagtgg
       61 atcctcagtc ctcgttgtgg ctggtgatgt ccagcttgat gtccacgttg taggcgccgg
      121 gcagctgcac gggcttcttg gccttgtagg tggtcttcac ctcggcgtcg tagtggccgc
      181 cgtccttcag cttcagtctc tggttgatct cgcccttcag ggcgccgtcc tcggggtaca
      241 ttctctcggt gctggcctcc cagcccatgg tcttcttctg catcacgggg ccgtcgctgg
      301 ggaagttggt gcccagcagc ttcaccttgt agatgaactc gccgtcctgc aggctgctgt
      361 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cactctctcc caggtgaagc
      421 cctcggggaa gctcagcttc aggtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      481 tgctgccgta catgaactgg gggctcagga tgtcccaggc gaagggcagg gggccgccct
      541 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gtggccctcg ccctcgccct
      601 cgatctcgaa ctcgtggccg ttcacgctgc cctccatgtg caccttgaat ctcatgaact
      661 ccttgatgat ggccatgttg tcctcctcca tggtaccaag cttaagttta aacgctagcc
      721 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
      781 tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      841 tt
//
