LOCUS       sequence-129954-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHRm-NLS-dCas9-GFP11x7-NLS
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      285..294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(343..363)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(379..589)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      385..520
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(439..458)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gctgtcgtcc accttggcca tctcgttgct gaagatctct tgcagatagc agatccggtt
       61 cttccgtctg gtgtatcttc ttctggcggt tctcttcagc cgggtggcct cggctgtttc
      121 tccgctgtcg aacagcaggg cgccgatcag gttcttcttg atgctgtgcc ggtcggtgtt
      181 gcccagcacc ttgaatttct tgctgggcac cttgtactcg tcggtgatca cggcccagcc
      241 cacagagttg gtgccgatgg ccaggccgat gctgtacttc ttgtccatgg tggcgatccc
      301 gggcccgcgg taccgcaagc ttgatatcct gcagacgcgt cccaccttgc gcttcttctt
      361 gggcatggtg gcgcgcgtag cctaggcctc caaaaaagcc tcctcactac ttctggaata
      421 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      481 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      541 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgcc tgaattcgat
      601 atcaagctta tcgatccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg
      661 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
      721 tattctttcc cctgcactgt acc
//
