LOCUS       sequence-129955-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP(1-10)-VP64-NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHRm-GFP(1-10)-VP64-NLS
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             416..565
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             593..613
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(729..749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tgactttttt aagtcggcta tgcctgaagg ttacgtgcaa gaaagaacaa tttcgttcaa
       61 agatgatgga aaatataaaa ctagagcagt tgttaaattt gaaggagata ctttggttaa
      121 ccgcattgaa ctgaaaggaa cagattttaa agaagatggt aatattcttg gacacaaact
      181 cgaatacaat tttaatagtc ataacgtata catcactgct gataagcaaa agaacggaat
      241 taaagcgaat ttcacagtac gccataatgt agaagatggc agtgttcaac ttgccgacca
      301 ttaccaacaa aacaccccta ttggagacgg tccggtactt cttcctgata atcactacct
      361 ctcaacacaa acagtcctga gcaaagatcc aaatgaaaaa ggtttaaacg gtagtgatgc
      421 tttagacgat tttgacttag atatgcttgg ttcagacgcg ttagacgact tcgacctaga
      481 catgttaggc tcagatgcat tggacgactt cgatttagat atgttgggct ccgatgccct
      541 agatgacttt gatctagata tgctaggtag tactagtggt ggcggcggaa gtcccaagaa
      601 gaagcgcaag gtgtaagcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat
      661 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      721 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
      781 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
      841 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
      901 gacgcaac
//
