LOCUS       sequence-129956-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP(1-10)-VP64-NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHRm-GFP(1-10)-VP64-NLS
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(254..661)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 ggccatggta caggaagttt accagtagta cagatgaatt taagagtcaa ttttccaatg
       61 gtggcgtcac cttctccttc gcctctcact gagaacttat gtccgttgac atcaccatcg
      121 agttcaacca aaattggcac aacaccggta aacagttctt ctcctttgga catggtggcg
      181 gatcccgggc ccgcggtacc gcaagcttga tatcctgcag acgcgtgaga attctcgaga
      241 gctccagatc ccccccgggc gactcagtct gtcggaggac tggcgcgccg agtgaggggt
      301 tgtgagctct tttatagagc tcgggaagca gaagcgcgcg aacagaagcg agaagcaggc
      361 tgattggtta attcaaataa ggcgcagggt catttcaggt ccttggggga gcctggaaac
      421 atctgatggg tcttaagaaa ctgctgaggg ttgggccata tctggggacc atctgttctt
      481 ggcctcgggc cggggccgaa actgcggtga ccatctgttc ttggccccgg gccggggccg
      541 aaactgctca ccgcagatat cctgtttggc ccaacgttag ctattttcat gtacccgccc
      601 ttgatctgaa cttctctatt cttggtttgg tatttttcca tgccttgcaa aatggcgtta
      661 ctgcagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
      721 taaataaaat cttttatttt atcggggctg caggaattcg atatcaagct tatcgatcca
      781 aactggatct ctgctgtccc
//
