LOCUS       sequence-129985-        4983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dopa decarboxylase from pig.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Satoh Y, Tajima K, Munekata M, Keasling JD, Lee TS
  TITLE     Engineering of L-tyrosine oxidation in Escherichia coli and 
            microbial production of hydroxytyrosol.
  JOURNAL   Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. 
            Epub 2012 Aug 29.
  PUBMED    22948011
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymben.2012.08.002"; journalName: "Metab Eng"; date: 
            "2012-11"; volume: "14"; issue: "6"; pages: "603-10"
COMMENT     Sequence Label: pBbE1k-DDC
FEATURES             Location/Qualifiers
     source          1..4983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..84
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             85..1167
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(104..123)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1399..1404,1405..1421,1422..1428)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1436..1452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1556..1567
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2980..3051
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3067..3094
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3252..3840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3332..3351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3928..4022)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4053..4847)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4164..4183)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4774..4793
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
       61 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt
     1201 gatctggttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1261 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1321 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1381 atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg
     1441 agcggataac aatttcagaa ttcaaaagat ctaaggagga taacatatga acgcgagtga
     1501 ctttcgccgt cgtggtaaag aaatggtcga ttacatggca gattatctgg aagggattga
     1561 gggccgccag gtttatcctg acgttcaacc tggctacctg cgtccgctga ttccggctac
     1621 cgcgccacag gaaccggata ccttcgaaga cattctgcaa gatgttgaaa agatcatcat
     1681 gccgggtgtc acgcactggc atagcccgta cttctttgct tatttcccaa ctgcaagttc
     1741 ttatccggct atgctggcag atatgctgtg tggggccatt ggctgcattg gctttagttg
     1801 ggctgcatct ccggcctgta cggaactgga gacggtgatg atggattggc tgggcaaaat
     1861 gctgcaactg cctgaagcgt ttctggccgg cgaagctggt gaaggcggtg gtgtgatcca
     1921 aggcagcgcc agcgaagcca ccctggtagc actgctggct gcacgtacca aagtcgttcg
     1981 tcgtctgcaa gccgcgtctc cgggtctgac ccagggtgcc gtgctggaga aactggtcgc
     2041 ctacgcctct gatcaggctc attctagtgt cgagcgtgca gggctgatcg gtggtgtaaa
     2101 actgaaagcg attccgtctg acgggaaatt tgccatgcgt gcgagcgctc tgcaagaagc
     2161 gctggaacgc gataaggccg ccggcctgat cccgttcttt gttgtcgcca cgctggggac
     2221 aacatcttgc tgtagtttcg acaatctgct ggaggttggc ccgatctgcc acgaagaaga
     2281 catctggctg catgtagacg ccgcgtacgc ggggagcgcg tttatttgcc ctgaatttcg
     2341 ccatctgctg aacggcgtcg agttcgcgga cagtttcaac tttaacccgc ataaatggct
     2401 gctggtgaat ttcgactgta gcgcgatgtg ggtaaaacgt cgcaccgatc tgacaggggc
     2461 gttcaaactg gacccagtgt atctgaagca tagccaccaa gggagcggtc tgattactga
     2521 ttatcgccac tggcagctgc ctctgggtcg tcgctttcgt agcctgaaaa tgtggtttgt
     2581 gtttcgtatg tatggtgtga agggtctgca agcatatatc cgtaaacatg tgcagctgag
     2641 tcacgagttt gaggctttcg tgctgcaaga tccacgcttt gaggtgtgtg cggaggttac
     2701 tctgggtctg gtgtgtttcc gcctgaaggg ctctgatggc ctgaatgaag cgctgctgga
     2761 acgcatcaat agcgcgcgta aaattcatct ggtgccatgc cgtctgcgcg gtcagttcgt
     2821 actgcgcttt gcaatttgta gtcgcaaagt tgagagcggc cacgtacgcc tggcgtggga
     2881 acatattcgc ggcctggcgg cagagctgct ggcagcagaa gaaggtaagg cagaaattaa
     2941 gagctaagga tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag
     3001 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     3061 cacactggct caccttcggg tgggcctttc tgcgtttata cctagggcgt tcggctgcgg
     3121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3661 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3901 gggattttgg tcatgactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     3961 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     4021 tccaagcgag ctctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4081 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4141 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     4201 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4261 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
     4321 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4381 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4441 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4501 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4561 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4621 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     4681 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4741 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4801 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4861 catcctgtct cttgatcaga tcatgatccc ctgcgccatc agatccttgg cggcaagaaa
     4921 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     4981 tcc
//
