LOCUS       sequence-129986-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: Amp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_5_Amp
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             285..663
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(319..338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agatcgaagg tctcccgagc cctaaataca ttcaaatatg tatccgctca tgagacaata
       61 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      121 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      181 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      241 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      301 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      361 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      421 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      481 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      541 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      601 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      661 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      721 ctggatggag gcggata
//
