LOCUS       sequence-129990-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(51..74)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(249..268)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     518..536
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggagtggcgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
       61 ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      121 cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct tcagcctctg
      181 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
      241 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      301 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
      361 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
      421 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
      481 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt
      541 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
      601 cacggagccc tccatgtgca ccttgaagcg
//
