LOCUS       sequence-129991-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  jxOFF-post.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  TITLE     Cre-dependent OFF post-mGRASP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: paavCAG-JxOFF-post-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atccagaggt tgattatcga taagctgtac cgttcgtata gcatacatta tacgaagtta
       61 tagatctgat agcttactta tacagctcat ccatgccata caagaacagg tgatgccgac
      121 cttctgagcg ttcgtactgc tcgacgattg tgtagtcctc gttgtgactc gtgatgtcca
      181 acttggtgtc cacgtagtag tagccaggca gctgaaccgg ctttttggcc atatatatag
      241 ttttgaactc aaccaggtag tgcccgccat ctttgagttt aagcgcctga tggatttcgc
      301 ctttaagaac gccatcgcgg gggtacagtc gttctgtaga ggcctcccac cccatggtct
      361 tcttctgcat cacggggcca tcggggggaa aatttgttcc gcgcatcttc accttgtaaa
      421 tcagggtgcc gtcctgcaga gaggagtctt gcgtgacagt gaccagccca ccatcctcaa
      481 aattcattac tcgttcccac ttaaatcctt caggaaatga cagttttttg taatcaggta
      541 tatc
//
