LOCUS       sequence-129993-         526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation transfer plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, 
            Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, 
            Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini 
            DM, Root DE
  TITLE     A lentiviral RNAi library for human and mouse genes applied to an 
            arrayed viral high-content screen.
  JOURNAL   Cell. 2006 Mar 24. 124(6):1283-98.
  PUBMED    16564017
REFERENCE   2  (bases 1 to 526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Mar 24. 124(6):1283-98."
COMMENT     Sequence Label: pLKO.1 - TRC cloning vector
FEATURES             Location/Qualifiers
     source          1..526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccggtg tgttgtaaat gagcacacaa aatacacatg ctaaaatatt atattctatg
      241 acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat aattagaatt
      301 tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa cgctagtttt
      361 agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt gttcctaagc
      421 ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact ccaccagctg
      481 ctttaactaa agcattgtct ttaacaactg acttcattag tttaac
//
